TAMPA, Fla. (WFLA) — Britain’s Queen Elizabeth II has died at her estate in Scotland, after officials with Buckingham Palace signaled earlier in the day that doctors were “concerned for Her Majesty’s health.”

“The Queen died peacefully at Balmoral this afternoon,” the royal family announced in an official statement on Thursday.

Buckingham Palace had previously announced that Elizabeth, 96, was under round-the-clock care at Balmoral Castle. Members of the royal family, including the Queen’s son, Prince Charles, his wife Camilla and the Queen’s grandson, Prince William, made their way to Scotland to be by the queen’s side.

Charles, meanwhile, is expected to be coronated as the next monarch in the coming days, according to the line of succession. In that line, Charles is followed by his eldest son, Prince William, Duke of Cambridge. Prince George is third in line to the throne, after his father and grandfather.

The royal family’s official statement, meanwhile, has already addressed Charles as king, and his wife, Camilla, as queen consort.

The royals have made a number of changes to succession rules over the years, shifting away from previous traditions.

Up until Duchess Kate’s pregnancy, the crown was passed from monarch to eldest son. Since King George VI had no son, his eldest daughter, Elizabeth, became queen.

But the Succession to the Crown Act was created in 2013, before Prince George was born, to make succession to the throne based on birth order, not gender.

Now, Princess Charlotte will be fourth in line to the throne, and Prince Louis will be fifth. William’s younger brother, Prince Harry, is sixth in line, and his son, Archie Harrison Mountbatten-Windsor, will be seventh with his little sister, Lilibet, right behind him.

A number of others are in line to claim the throne. Here are the 25 in the line of succession after Elizabeth:

  1. Charles, Prince of Wales – married to Camilla, Duchess of Cornwall
  2. Prince William, Duke of Cambridge – married to Kate, Duchess of Cambridge
  3. Prince George of Cambridge
  4. Princess Charlotte of Cambridge
  5. Prince Louis of Cambridge
  6. Prince Harry, Duke of Sussex – married to Meghan, Duchess of Sussex
  7. Archie Harrison Mountbatten-Windsor
  8. Lilibet Diana Mountbatten-Windsor
  9. Prince Andrew, Duke of York – Queen Elizabeth’s second son
  10. Princess Beatrice of York – Prince Andrew and Sarah, Duchess of York’s daughter
  11. Sienna Elizabeth Mapelli Mozzi, Princess Beatrice’s daughter
  12. Princess Eugenie of York – Prince Andrew and Sarah, Duchess of York’s daughter
  13. August Philip Hawke Brooksbank, Princess Eugenie’s daughter
  14. Prince Edward, Earl of Wessex – Queen Elizabeth’s third son
  15. James, Viscount Severn – Prince Edward and Sophie, Countess of Wessex’s son
  16. Lady Louis Windsor – Prince Edward and Sophie, Countess of Wessex’s daughter
  17. Anne, Princess Royal – Queen Elizabeth’s daughter
  18. Peter Phillips – Princess Anne’s son with Capt. Mark Phillips
  19. Savannah Phillips – Peter and wife Autumn’s daughter
  20. Isla Phillips – Peter and wife Autumn’s daughter
  21. Zara Tindall – Zara Phillips married Mike Tindall, the eldest granddaughter of Queen Elizabeth
  22. Mia Grace Tindall – Zara and Mike’s daughter
  23. Lena Tindall, Zara and Mike’s daughter
  24. Lucas Tindall, Zara and Mike’s daughter
  25. David Armstrong-Jones, 2nd Earl of Snowden – Also known as Viscount David Linley 

Prior to her death, Elizabeth had increasingly handed over duties to Charles and other members of the royal family, including her usual duty of presiding over the State Opening of Parliament. Charles had also been sharing the queen’s official duties for several years in preparation for his eventual ascension to the throne.